Medico-Legal Update

Hibah Hasan Ibrahim ¹, Mutaz Sabah Ahmeid ², Nihad Khalawe Tektook ³, Hashim Abdulsattar Jabar ⁴

Affiliations
1 Kirkuk Health Directorate, Kirkuk City, IQ
2 M.B.Ch.B, M.Sc, Ph.D in Clinical Biochemistry, College of Medicine, Tikrit University, IQ
3 Ph.D in Immunology, Middle Technical University, College of Medical & Health Technology, Baghdad, IQ
4 B.Sc, M.Sc in Chemistry, Department of Biochemistry, College of Medicine, Tikrit University, IQ

Abstract

Background: Klotho, is a protein associated with life extension plays a important role in kidney disease progression, anti-aging, anti-oxidation, modulation of ion transport, and development of disturbed mineral metabolism. The aim of the study was to measure Soluble α-Klotho(SAKL) levels in chronic kidney disease(CKD) patients before and after dialysis in relation with Lipid profile. Methods: This shortprospective hospital-based study was done in the Department of Chemistry and Biochemistry, College of medicine, Tikrit University, Tikrit, Iraq. The study was carried out for 30 patients subjected to Hemodialysis recruited from Tikrit Teaching Hospital, hemodialysis unit between 1st December, 2018 and 1st April, 2019. The study also included 30 adult persons looks healthy with no prior medical or family history of CKD as a control participated in this study. The levels of SAKL and lipid profile were measured in the serum of 30 patients, before and after Hemodialysis and compared with controls. Results: The study revealed increased in the SAKL level in CKD patients before dialysis compared to healthy control group, and decreased in group of CKD after dialysis compared to control group. Furthermore, there was a significant positive correlation of SAKL level with serum Triglyceride and Very low density lipoprotein cholesterol(VLDL-C) in CKD patients before dialysis. Conclusions: There was a highly significant relation of SAKL with lipid abnormality in CKD patients under hemodialysis.

Keywords

Soluble α,-Klotho, Chronic Kidney Disease, Hemodialysis, Lipid Profile.

Full Text

     

Asaal Abdul-Ghani Shyeaa ¹, Mohammed Abdul-Daim Saleh ², Nihad Khalawe Tektook ³

Affiliations
1 Department of Biology, College of Science, Diyala University, Diyala, IQ
2 Department of Biotechnology, College of Science, Diyala University, Diyala, IQ
3 College of Health and Medical Technics, Middle Technical University, Baghdad, IQ

Abstract

Objectives: The present study designed to investigate the Immunological role of toll-like receptor-7, IL-17A and IL-10 in sera of 66 Chronic Renal Failure CRF patients at IBN-SINA center for Dialysis in Diyala governorate - Iraq. Methods: Sandwich Enzyme Linked Immuno-Sorbent Assay(ELISA) technique were used in this study to evaluate concentration of TLR-7 ,IL-17A and IL-10 between study groups. Results: The findings of the present study demonstrated that significantly increased level of TLR-7 in the CRF group (0.24± 0.05 ng/ml) compared to controls (0.02± 0.08 ng/ml), the findings revealed a significant association between CRF and IL-17A serum level in the studied groups,There was a significantly increased level of IL- 17A in the CRF group (6.50±1.37 pg/ml) compared to controls (2.19± 0.32 pg/ml) also decreased level of IL-10 in the CRF group (26.20± 5.16 pg/ml) compared to controls (79.27±23.44 pg/ml). Conclusion: These findings concluded that TLR-7, IL-17A and IL-10 might have a role in CRF pathogenesis.

Keywords

Chronic Renal Failure, TLR-7, IL-17A, IL-10, Pro-Inflammatory Cytokines.

Full Text